Journal: PLoS ONE

Article Title: Improving Osteoblast Response In Vitro by a Nanostructured Thin Film with Titanium Carbide and Titanium Oxides Clustered around Graphitic Carbon

doi: 10.1371/journal.pone.0152566

Figure Lengend Snippet: Panel a: The Saos-2 (left panels) and the hOB cells (right panels) were grown for 96 h on glass slides coated with 10.5 nm of titanium or the nanostructured TiC layer, treated with primary monoclonal antibodies against integrin α3β1 (10 μg/ml). Panel b: The cells were treated with primary monoclonal antibodies against talin (10 μg/mL). Panel c: Cells were treated with primary monoclonal antibodies against paxillin (10 μg/ml). In all cases, the treatment was followed with Alexa Fluor 568 goat anti-mouse secondary antibodies, diluted 1:500 and the nuclei were stained with DAPI. The images were collected with a magnification of 63X for ITGA and PAX and of 100x for TAL, and the bar represents 100 μm.

Article Snippet: Anti-paxillin rabbit polyclonal antibodies (NBP1-19833) and anti-talin mouse monoclonal antibodies (NBP1-21642) were obtained from Novus Biologicals (Littlenton, CO, USA).

Techniques: Bioprocessing, Staining

Journal: Journal of Cancer

Article Title: Inward Tension of Talin and Integrin-related Osmotic Pressure are involved Synergetically in the Invasion and Metastasis of Non-small Cell Lung Cancer

doi: 10.7150/jca.45494

Figure Lengend Snippet: Talin-1 rather than talin-2 is associated with malignancy in NSCLC ( A ) Kaplan-Meier plots of overall survival of patients with squamous carcinoma and adenocarcinoma, stratified by talin-1 and talin-2 expression. ( B ) Western blotting of talin protein levels in fresh NSCLC tissue ( C ) and adjacent normal tissue (P). (C) Normalized quantitative analysis of the wound closure rate in A549 cells transfected with control siRNA or talin-1 siRNA. ( D ) Quantitative analysis of the migration and invasion rates. **P <0.01 compared with the control group, ***P <0.001 compared with the control group.

Article Snippet: Antibody against talin (A02859-1, Western blotting 1:500) was purchased from Boster Bio (Pleasanton, CA, USA).

Techniques: Expressing, Western Blot, Transfection, Control, Migration

Journal: The Journal of Cell Biology

Article Title: Focal adhesions are essential to drive zebrafish heart valve morphogenesis

doi: 10.1083/jcb.201807175

Figure Lengend Snippet: Localization of factors involved in cytoskeletal organization in migrating AV ECs. (A–H) Immunohistochemistry of 58-hpf Tg(nfatc1:GAL4); Tg(UAS:EGFP-CAAX) (A–F) or Tg(nfatc1:GAL4); Tg(UAS:EGFP) (G and H) embryos. Green, GFP; cyan, DAPI; magenta (A–H) and white (A′–H′), specific factors of interest. (A–F) Localization of FA-associated factors. (A and B) Itgβ1 (A) and Itgα5 (B) appeared enriched in discrete puncta at the migrating cell protrusions. (C–E) Cytoplasmic FA adaptor molecules Talin1 (C), Vinculin (D), and p-Pax (E) appeared strongly localized in the leading edge of migrating cells. (F) The Itgα5β1 ligand Fn appeared distributed around the endocardial and myocardial cells at the AVC. (G and H) The tight junction–associated protein ZO-1 and adherens junction protein VE-cadherin were localized between the leading and follower AV ECs but appeared undetectable in the EC protrusions. Dashed red lines outline the leading edge of the migrating cells at a distance of 1–2 µm. Scale bars: (A–F) 20 µm; (A′–F′ and G–H′) 10 µm.

Article Snippet: Primary antibodies (used at 1:500 dilution) were Itgα5 (rabbit, STJ97284; Antibodyplus), Itgβ1 (rabbit, STJ93731; Antibodyplus), Talin1 (rabbit, STJ97271; Antibodyplus), Vinculin (rabbit, V4139; Sigma-Aldrich), p-Pax (rabbit, NPB2-24459; Novus Biologicals), Fn (rabbit, F3648; Sigma-Aldrich), ZO-1 (mouse, 33-9100; Thermo Fisher Scientific), GFP (chicken, ab13970; Abcam), and mCherry (ab125096; Abcam).

Techniques: Immunohistochemistry